![]() ![]() Overall, 443 exosomal proteins, including 110 differentially expressed proteins (DEPs) were identified by quantitative proteomics analyses. However, the exosomal negative marker calnexin, an endoplasmic reticulum protein, was not detected in exosomes. The exosomal markers CD9 and CD81 were observed in the serum exosomes. The size of the serum derived exosomes ranged from 30 to 150 nm in diameter. Results: We isolated exosomes from serum samples. Finally, the expression of exosomal protein candidates was selectively validated in individual subjects by western blot analysis. ![]() Next, quantitative proteomic analyses were applied to analyze the protein profiles of the exosomes, and bioinformatic analyses were conducted on the proteomic data. Methods: Exosomes were isolated from pooled serum samples of 20 mGC patients and 40 healthy controls (HC) by ultracentrifugation. Therefore, in this study, we aimed to reveal the proteomic profile of mGC-derived exosomes. Recently, accumulating evidence has suggested that exosomes play an essential role in cancer metastasis and can be an excellent reservoir of novel biomarkers and candidate therapeutic targets for cancer. Faculty of Medical Laboratory Science and Central Laboratory, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, Chinaīackground: Clinical management of metastatic gastric cancer (mGC) remains a major challenge due to a lack of specific biomarkers and effective therapeutic targets.For more details, see: Recommendations for Test Performance and Interpretation from the Second National Conference on Serologic Diagnosis of Lyme Disease.Xiao-Qing Ding Zhe-Ying Wang Di Xia Rui-Xian Wang Xiao-Rong Pan * Jian-Hua Tong * Before CDC will recommend new tests, they must be cleared by the Food and Drug Administration (FDA). New tests may be developed as alternatives to one or both steps of the two-step process. Positive IgM results should be disregarded if the patient has been ill for more than 30 days.ĬDC supports the development of new tests ![]()
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